PE-SB

• For detail, see our manuscript : AI-generated small binder improves prime editing
• BAE Lab website

1. Generate 'Binder Protein' easily via Google Colab without Local GPU.

• BAELAB_RFdiffusion_batch_process.ipynb - Run BAELAB batch code for RFdiffusion to generate SB(small binder) in Google Colab.

2. Analyse result files via Matlab code.

• RFdiffusion_result_analysis.m - Download and run BAELAB analysis code to analyse result files from RFdiffusion and AF3 in Matlab.

3. Use AlphaFold 3 website to do a competition test. Details in our manuscript.

• AlphaFold3 website - You can use json files generated by Matlab code.

4. Analyse result files again via Matlab code with AF3 competition data.

5. Validate the SB in experiment!

Data : PE-SB

• PE-SB_ExperimentCandidates.zip - Download and use PE-SB data as example data.