descSPIM-basic

Introduction

The descSPIM project aims to build an open-source microscopy platform that can be built with low cost and low expertise in optics.

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The main features of the descSPIM-basic setup are:

● Compatibility with major tissue clearing technologies, including CUBIC, BABB, 3DISCO/iDISCO, and SHIELDS.

● The construction can be completed in half a day.

● It does not require a high level of microscopy expertise.

● All parts are commercially available (mostly from Thorlabs).

● An affordable cost ($20k-50k) is achieved.

● A volume imaging completing within a few minutes.

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Parts

The above image and a breadboard of 30 cm x 45 cm are all the components needed to build descSPIM. A photo of the completed instrumentation is in Introduction. The 3D CAD of the finished drawing is in README.md.

Parts list

The parts list for descSPIM-basic can be downloaded here.

You can order all the parts (except for laser) to Thorlabs.

Construction

How to construct descSPIM

descSPIM is a light-sheet microscope that you can build as if you were assembling a block toy. A construction tutorial is provided as follows. You can use the guide throughout the construction procedure.

Below is the manual for the construction procedure.

construction manual ver. 230520.

opt. alignment manual ver. 230520.

Operation

This section summarizes the operation of descSPIM. Please refer to the following PDF file Explanation MOVIE is also available.

3D imaging manual ver. 230520.

(tutrial movie: coming soon)

Introduced sites

Here is a list of sites related to descSPIM. Please check them.

(coming soon)