Romanowsky Stain Slide Analyzer for Windows

March 24, 2026 · View on GitHub

Romanowsky Stain Slide Analyzer for Windows

Segmentation & Labeling Tool for Data Learning and Verification Exclusive to Romanowsky Stain Slide Analyzer
ⓒ 2024-2025 Changjin Ha. All Rights Reserved.

🚀 Tech Stack

Client (Windows)

UI Framework: WinUI3 (.NET 9) / Windows App SDK
Language: C#

AI & Inference Environment (WSL2)

AI Model: Meta SAM2 (Segment Anything Model 2)
Environment: Ubuntu (Windows Subsystem for Linux 2) / Python venv
Hardware Acceleration: PyTorch with Multi-GPU selection support

Bridge & IPC (Inter-Process Communication)

Execution: Subprocess spawning (ubuntu run ...)
Data Exchange: File-system based IPC (Read/Write via shared Windows directory)

🏗️ Architecture

graph TD
    %% Shared File System (Bridge)
    subgraph SharedFS [📁 Shared Directory IPC]
        Image[Original Slide Image]
        CSV[Coordinates & Mask CSV]
    end

    %% WSL Backend
    subgraph WSLGuest [🐧 WSL 2: AI Backend]
        SAM2[Meta SAM2 Model / Multi-GPU]
        PythonEnv[Python Subprocess]
        
        PythonEnv -->|1. Load Image| Image
        PythonEnv -->|2. Batch Inference| SAM2
        SAM2 -->|3. Extract All Masks| CSV
    end

    %% Windows Frontend
    subgraph WindowsHost [🖥️ Windows Host: WinUI 3]
        UI[Interactive UI & Viewer]
        SubProcess[Trigger `ubuntu run`]
        InMemory[Local Memory / State]

        %% Phase 1: Init Flow
        UI -->|0. Select File| SubProcess
        SubProcess -->|Execute Once| PythonEnv
        
        %% Phase 2: Interaction Flow
        CSV -->|4. Parse to RAM| InMemory
        UI <-->|5. Point-click, Color Analysis| InMemory
    end

🧱 If I were to rebuild it in 2026

Layer	Original	2026 Pick	Reason
ML inference	Python/SAM2 via WSL subprocess	ONNX Runtime + DirectML	Native GPU, no WSL, no subprocess
IPC (if keep Python)	`ubuntu run {cli_string}`	FastAPI + `HttpCLient.PostAsJsonAsync`	Typed JSON, async, no CLI injection
Settings storage	`Local Settings` string keys	`LocalSettings` + typed constants class	Same API, add compile-time safety
Preset storage	`.txt` with `Split(": ")` parser	`System.Text.Json` + typed POCO	Robust, no custom parser
Mask storage	CSV (0/1 text)	NPY or PNG	30-50x smaller, standard tooling
WSL detection	WMI `Win32_OptionalFeature`	`wsl --status` subprocess	Simpler, locale-independent
GPU enumeration	NvAPIWrapper + WMI redundancy	NvAPIWrapper only (or DirectML device enum)	One path

✨ Core Features

Show Contents

Segmentation

Just load the images, name the file, and segmentation is complete.

You can select GPU to segment.

Also, you can use GPU Parallel for use multiple GPUs.

And you can choose model to use.

Customize Parameters

If you proceed with Automatic Segmentation, you can also modify SAM parameters.

You can also save presets and use them again later.

Select Points

Segment only the parts you want accurately through Manual Segmentation.

Segmentation Result

Just wait a moment and the segmentation results will be right before your eyes.

Labeling

If you selected the Extract Bounding Boxes or Extract Masks option, try labeling using the Labeling function.

Export

From labeling to exporting, all in one go.

History

A strong assistant that not only checks and saves past results, but also replaces and exports labeling data, and analyzes and saves bounding boxes.

Image Viewer

A simple yet powerful tool to view, zoom, rotate and save images.

Analyze

Once labeling is complete, try using the Analyze function, which analyzes ARGB, Hue, Saturation, and Brightness by class.

Export

Of course, the Export function is built in as standard.

Settings

A smart friend that checks everything from history initialization to plugin version management and even environment status.

Configure Environment

A solid feature that handles all environments at once, from checking WSL status to installation, Linux installation, SAM download, CUDA and cuDNN, Python installation, and even copying entry points.

Feature Activator

Are you having trouble installing Windows Additional Features and Linux? Feature Activator takes care of it all.

Compatibility

Romanowsky Stain Slide Analyzer for Windows is compatible with these devices.

	Minimum Requirements	Recommended Requirements
CPU	7th Gen. Intel Core i3	9th Gen. Intel Core i7
RAM	8GB	16GB
GPU	None	NVIDIA Geforce RTX 3060
Operating System	Windows 10 (x64, 22H2)	Windows 11
Storage	4GB HDD	8GB SSD

📝 Release Notes

Show Contents

Release Note

Here are the release notes for each version of Romanowsky Stain Slide Analyzer for Windows.

Romanowsky Stain Slide Analyzer 1.4.0.0 Release Note

Framework

Saving a single file now displays the File Save Dialog instead of the Folder Picker, allowing you to customize the file's name and extension.

Segmentation

Newly designed HomeView.

You can now add files by dragging.

You can now segment multiple images.

You can now choose which GPU to use for inference.

Parallel GPU option is now available.

Shortcuts have been applied to some functions.

The ability to cancel segmentation is now available.

You can now apply the currently set options to all files.

Model Selection is now available.

Customize Parameters

Parameter Preset is now available.

Number Box is now available.

History

The History list is now displayed by file name.

Shortcuts have been applied to some functions and menus.

Image Viewer is now available.

Calendar Date Picker is now available.

Labeling

Now you can check the mask and bounding box at the same time.

Shortcuts have been applied to some functions.

Fixed the issue where Bounding Box indexes were not displayed completely.

Analyze

You can now check mask data and bounding box data at the same time.

Shortcuts have been applied to some functions.

Settings

You can now delete the Preset.

Romanowsky Stain Slide Analyzer 1.3.0.0 Release Note

Segmentation

Extract Masks is now available.

An option is available to save the image immediately after segmentation is complete.

History

A completely new design for History View is now available.

Re-labeling is now available.

Save Mask Labeling Data is now available.

Label Mask Data is now available.

Save the masked image is available.

Labeling

Toggle Bounding Box / Mask option is now available.

Now when you click the back and next buttons, if there is any saved data it will be displayed.

Analyze

The ability to analyze segmentation mask data is now available.

Toggle Bounding Box / Mask option is now available.

Fixed the issue where the average A value was not displayed properly when exporting data.

Romanowsky Stain Slide Analyzer 1.2.1.0 Release Note

Feature Activator

Fixed an issue where environment configuration was not performed properly on Windows 10.

Framework

Improved environment configuration and segmentation to allow for drives other than C drive.

Romanowsky Stain Slide Analyzer 1.2.0.0 Release Note

Select Points

Fixed an issue where points were not displayed properly in Manual Segmentation mode

History

Labeling Data Change is now available.

Analyze Bounding Box is now available.

Export Image with Bounding Boxes is now available.

Labeling

Toggle Bounding Box Show/Hide is now available.

Fixed an issue where blank spaces were appearing at the beginning of each piece of data.

Automatic Zoom & Scroll is now available.

Fixed an issue where labeling would be reset even if the Cancel button was pressed in the dialog that appears when there are already labeled files.

Analyze

The Analyze feature is now available to view and export Color, Hue, Saturation, Brightness by Bounding Box and the overall Color, Hue, Saturation, Brightness average.

Romanowsky Stain Slide Analyzer 1.1.0.0 Release Note

Segmentation

Manual Segmentation is now available.

Extract Bounding Boxes option is now available for extracting bounding box coordinates.

Labeling is now available.

Select Points

You can segment only that part by clicking on the part you want to segment with the mouse.

You can specify the class of the area to be segmented.

Labeling

You can specify a class for each bounding box area.

You can export the labeled file to a csv file.

History

You can save the result image file.

You can re-save the labeled csv file.

Fixed an issue where records would not display properly when the date was changed.

Records are now displayed in most recent order.

Settings

You can check and update each library version.

Romanowsky Stain Slide Analyzer 1.0.0.0 Release Note

Segmentation

Automatic Segmentation is now available.

Customize Parameters is now available.

History

History is now available.

Settings

Check Environment configure status is now available.

Environment

Romanowsky Stain Slide Analyzer Feature Activator is now available.

Automatic configure environment is now available.