CB321qc

Learning objectives

• Describe sequencing by synthesis, including cDNA library preparation.
• Describe the design considerations to perform an RNA sequencing experiment.
• Describe the workflow for a standard bulk or single-cell RNA-seq data analysis.
• List computational skills necessary to implement the various steps in the analysis workflow.
• List genomics-specific databases for data sharing and download

Lesson Plan

1. Introduction
2. RNA-seq library prep and sequencing basics
3. Bulk RNA-seq
   1. Experimental design
   2. Analysis
   3. Visualization of DE results
   4. Functional analysis
4. Single Cell RNA-seq
5. Types of genomics data
6. Data access and sharing
7. Data storage

Resources

• Planning a successfull bulk RNA-seq experiment
• "How many cells are needed per sample for my single-cell experiment?"