README.md

Description

These lessons are adapted from a tutorial presented by the HBC training team at the 2018 GCC/BOSC conference. The adaptation is for a 3 hour presentation about RNA-seq (bulk & single cell) experimental design for CELLBIO 321qc.

Instructors and contributors

Instructor 1: Dr. Sarah Boswell, Laboratory of Systems Pharmacology, Single Cell Core

Instructor 2: Dr. Radhika Khetani, Training Director at the Harvard Chan Bioinformatics Core

Contributors: Dr. Mary Piper and Dr. Meeta Mistry, Harvard Chan Bioinformatics Core

Schedule

Time	Topic	Instructor
9:00 - 9:10	Introduction to module	Radhika & Sarah
9:10 - 9:25	Sequencing by synthesis	Sarah
9:25 - 9:50	Experimental design for RNA-seq	Radhika
9:50 - 10:35	RNA-seq library preparation	Sarah
10:35 - 10:45	Break
10:45 - 11:05	Practical strategies for sequencing	Radhika & Sarah
11:05 - 11:40	Analysis workflow and tools (bulk RNA-seq)	Radhika
11:40 - 11:50	Analysis workflow and tools (single-cell RNA-seq)	Radhika
11:50 - 12:00	Wrap-up	Sarah & Radhika